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Loss of monocarboxylate transporter 1 aggravates white matter injury after experimental subarachnoid

《医学前沿（英文）》 2021年第15卷第6期页码 887-902 doi: 10.1007/s11684-021-0879-9

摘要： Monocarboxylic acid transporter 1 (MCT1) maintains axonal function by transferring lactic acid from oligodendrocytes to axons. Subarachnoid hemorrhage (SAH) induces white matter injury, but the involvement of MCT1 is unclear. In this study, the SAH model of adult male Sprague-Dawley rats was used to explore the role of MCT1 in white matter injury after SAH. At 48 h after SAH, oligodendrocyte MCT1 was significantly reduced, and the exogenous overexpression of MCT1 significantly improved white matter integrity and long-term cognitive function. Motor training after SAH significantly increased the number of ITPR2⁺SOX10⁺ oligodendrocytes and upregulated the level of MCT1, which was positively correlated with the behavioral ability of rats. In addition, miR-29b and miR-124 levels were significantly increased in SAH rats compared with non-SAH rats. Further intervention experiments showed that miR-29b and miR-124 could negatively regulate the level of MCT1. This study confirmed that the loss of MCT1 may be one of the mechanisms of white matter damage after SAH and may be caused by the negative regulation of miR-29b and miR-124. MCT1 may be involved in the neurological improvement of rehabilitation training after SAH.

关键词： microRNAs monocarboxylate transporter 1 motor training subarachnoid hemorrhage white matter injury

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Cotransfecting norepinephrine transporter and vesicular monoamine transporter 2 genes for increased retention

null

《医学前沿（英文）》 2017年第11卷第1期页码 120-128 doi: 10.1007/s11684-017-0501-3

摘要：

Norepinephrine transporter (NET) transfection leads to significant uptake of iodine-131-labeled metaiodobenzylguanidine (¹³¹I-MIBG) in non-neuroendocrine tumors. However, the use of ¹³¹I-MIBG is limited by its short retention time in target cells. To prolong the retention of ¹³¹I-MIBG in target cells, we infected hepatocarcinoma (HepG2) cells with Lentivirus-encoding human NET and vesicular monoamine transporter 2 (VMAT2) genes to obtain NET-expressing, NET-VMAT2-coexpressing, and negative-control cell lines. We evaluated the uptake and efflux of ¹³¹I-MIBG both in vitro and in vivo in mice bearing transfected tumors. NET-expressing and NET-VMAT2-coexpressing cells respectively showed 2.24 and 2.22 times higher ¹³¹I-MIBG uptake than controls. Two hours after removal of ¹³¹I-MIBG-containing medium, 25.4% efflux was observed in NET-VMAT2-coexpressing cells and 38.6% in NET-expressing cells. In vivo experiments were performed in nude mice bearing transfected tumors; results revealed that NET-VMAT2-coexpressing tumors had longer ¹³¹I-MIBG retention time than NET-expressing tumors. Meanwhile, NET-VMAT2-coexpressing and NET-expressing tumors displayed 0.54% and 0.19%, respectively, of the injected dose per gram of tissue 24 h after ¹³¹I-MIBG administration. Cotransfection of HepG2 cells with NET and VMAT2resulted in increased ¹³¹I-MIBG uptake and retention. However, the degree of increase was insufficient to be therapeutically effective in target cells.

关键词： norepinephrine transporter vesicular monoamine transporter 2 -MIBG gene therapy lentivirus vector

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Expression and function of DMT1 without IRE in C6 cells mediated by recombinant adenovirus

Xixun DU*, Huamin XU*, Hong JIANG, Jun WANG, Lei WANG, Junxia XIE

《医学前沿（英文）》 2009年第3卷第1期页码 67-71 doi: 10.1007/s11684-009-0010-0

摘要： Divalent metal transporter 1 (DMT1) is a ferrous iron import protein. The improper expression of DMT1 is involved in neurodegenerative diseases. In the present study, we constructed a recombinant adenovirus containing the gene of DMT1 without the iron response element (DMT1-IRE) and investigated its expression and function in the C6 glioma cell line. The DMT1-IRE gene, obtained by RT-PCR, was cloned into the shuttle plasmid pAdTrack-CMV containing green fluorescent protein (GFP) reporter gene. Linearized plasmid pAdTrack-CMV-DMT1-IRE was subsequently co-transformed into ( ) BJ5183 cells along with an adenoviral backbone plasmid pAdEasy-1 after digestion with I. I-digested pAdEasy1-DMT1-IRE was then transfected into E1-transformed human embryonic kidney cells (HEK293 cells) , in which recombinant adenoviruses were generated within 7 to 10 days. The results demonstrated that we obtained the DMT1-IRE gene. pAdEasy1-DMT1-IRE yielded a large fragment, plus a smaller fragment of 4.5 kb after digestion with I. PCR confirmed pAdEasy1-DMT1-IRE contained gene DMT1-IRE, indicating the successful construction of recombinant adenovirus plasmid containing DMT1-IRE. GFP fluorescence further confirmed the generation of adenovirus. AdDMT1-IRE could efficiently infect C6 glioma cells. And cell viability decreased in Ad-DMT1-IRE infected cells after iron overload compared to the control. These results suggest that the over expressed DMT1-IRE can aggravate the iron induced cell death due to its iron influx function.

关键词： divalent metal transporter 1 recombinant adenovirus homologous recombination iron

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a novel K+ transporter gene in cotton

Yiru WANG, Juan XU, Mingcai ZHANG, Xiaoli TIAN, Zhaohu LI

《农业科学与工程前沿（英文）》 2018年第5卷第2期页码 226-235 doi: 10.15302/J-FASE-2017170

摘要： Potassium is an essential nutrient for plant growth and productivity of crops. K transporters are important for K uptake and transport in plants. However, information on the function of K transporters and K channels in cotton is limited. The KT/KUP/HAK protein family is essential for a variety of physiological processes in plants, including nutrient acquisition and regulation of development. This study, identified a K transporter gene, expressed in the roots of cotton ( ) cv. Liaomian17. The deduced transcript of is highly homologous to Cluster II of KUP/HAK/KT K transporters and is predicted to contain 11 transmembrane domains. has been localized to the plasma membrane, and its transcripts were detected in roots, stems, leaves and shoot apices of cotton seedlings. Consistently, b-glucuronidase (GUS) expression driven by the GhKT2 promoter could be detected in roots, mesophyll cells, and leaf veins in transgenic . In addition, the expression of was induced by low K stress in cotton roots and ::GUS-transgenic seedlings. The -overexpression lines plants were larger and showed greater K accumulation than the wild type (WT) regardless of K concentration supplied. The net K influx rate, measured by the noninvasive micro-test technique, in root meristem zone of -transgenic lines was significantly greater than that of WT. Taken together, this evidence indicates that GhKT2 may participate in K acquisition from low or high external K , as well as K transport and distribution in plants.

关键词： cotton GhKT2 potassium transporter uptake

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Identification of transporter proteins for PQQ-secretion pathways by transcriptomics and proteomics analysis

Hui Wan,Yu Xia,Jianghua Li,Zhen Kang,Jingwen Zhou

《化学科学与工程前沿（英文）》 2017年第11卷第1期页码 72-88 doi: 10.1007/s11705-016-1580-4

摘要： Pyrroloquinoline quinone (PQQ) plays a significant role as a redox cofactor in combination with dehydrogenases in bacteria. These dehydrogenases play key roles in the oxidation of important substrates for the biotechnology industry, such as vitamin C production. While biosynthesis of PQQ genes has been widely studied, PQQ-transport mechanisms remain unclear. Herein, we used both two-dimensional fluorescence-difference gel electrophoresis tandem mass spectrometry and RNA sequencing to investigate the effects of overexpression in an industrial strain of WSH-003. We have identified 73 differentially expressed proteins and 99 differentially expressed genes, a majority of which are related to oxidation-reduction and transport processes by gene ontology analysis. We also described several putative candidate effectors that responded to increased PQQ levels resulting from overexpression. Furthermore, quantitative PCR was used to verify five putative PQQ-transport genes among different PQQ producing strains, and the results showed that , and were upregulated in all conditions. Then the three genes were over-expressed in WSH-003 and PQQ production were detected. The results showed that extracellular PQQ of B932_1930 (a transporter) and B932_2186 (an ABC transporter permease) overexpression strains were enhanced by 1.77-fold and 1.67-fold, respectively. The results suggest that the proteins encoded by PqqB, B932_1930 and B932_2186 might enhance the PQQ secretion process.

关键词： 2D-DIGE pqqB pyrroloquinoline quinone RNA-Seq Vitamin C

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灰色预测模型GM（１，１）的适用性分析及在火灾风险预测中的应用

陈子锦,王福亮,陆守香

《中国工程科学》 2007年第9卷第5期页码 91-94

摘要：

通过对灰色预测模型———GM（１，１）的理论分析，证明了该模型的预测值及其变化趋势均具有单调性，进而提出了GM（１，１）模型的适用性判据，并给出了该判据在火灾风险灰色预测中的应用实例。

关键词：火灾预测 GM(1 1)模型火灾伤人率

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Metabolic interventions combined with CTLA-4 and PD-1/PD-L1 blockade for the treatment of tumors: mechanisms

《医学前沿（英文）》页码 805-822 doi: 10.1007/s11684-023-1025-7

摘要： Immunotherapies based on immune checkpoint blockade (ICB) have significantly improved patient outcomes and offered new approaches to cancer therapy over the past decade. To date, immune checkpoint inhibitors (ICIs) of CTLA-4 and PD-1/PD-L1 represent the main class of immunotherapy. Blockade of CTLA-4 and PD-1/PD-L1 has shown remarkable efficacy in several specific types of cancers, however, a large subset of refractory patients presents poor responsiveness to ICB therapy; and the underlying mechanism remains elusive. Recently, numerous studies have revealed that metabolic reprogramming of tumor cells restrains immune responses by remodeling the tumor microenvironment (TME) with various products of metabolism, and combination therapies involving metabolic inhibitors and ICIs provide new approaches to cancer therapy. Nevertheless, a systematic summary is lacking regarding the manner by which different targetable metabolic pathways regulate immune checkpoints to overcome ICI resistance. Here, we demonstrate the generalized mechanism of targeting cancer metabolism at three crucial immune checkpoints (CTLA-4, PD-1, and PD-L1) to influence ICB therapy and propose potential combined immunotherapeutic strategies co-targeting tumor metabolic pathways and immune checkpoints.

关键词： CTLA-4 PD-1 PD-L1 immune checkpoint blockade (ICB) metabolic reprogramming combined tumor therapeutic strategies

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渤海辽东带地质认识的突破与金县1-1大油田的发现

邓运华

《中国工程科学》 2011年第13卷第10期页码 13-18

摘要：在此认识指导下选择研究方向，评价有利目标，经钻探发现了金县1-1大油田，储量达1.5×10⁸ m³，充分显示了科研在油气勘探中的作用。

关键词：渤海辽东带金县1-1 地质认识大油田

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联邦无监督表示学习 Research Article

张凤达1,况琨1,陈隆1,游兆阳1,沈弢1,肖俊1,张寅1,吴超2,吴飞1,庄越挺1,李晓林3,4,5

《信息与电子工程前沿（英文）》 2023年第24卷第8期页码 1181-1193 doi: 10.1631/FITEE.2200268

摘要： FURL提出了两个新挑战：（1）客户端之间的数据分布转移（非独立同分布）会使本地模型专注于不同的类别，从而导致表示空间的不一致；（2）如果FURL中客户端之间没有统一的信息，客户端之间的表示就会错位。

关键词：联邦学习；无监督学习；表示学习；对比学习

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非等间距GM(1,1)幂模型及其工程应用

王正新,党耀国,刘思峰

《中国工程科学》 2012年第14卷第7期页码 98-102

摘要：

针对工程中大量存在的非等间距序列的建模问题，提出了非等间距GM(1, 1)幂模型。以平均相对误差绝对值最小化为目标，以模型参数之间的关系为约束，构建了一个非线性优化模型实现非等间距GM(1, 1)幂模型的参数估计。结果表明，非等间距GM(1, 1)幂模型的形式较为灵活，非等间距GM(1, 1)模型和灰色Verhulst模型均是非等间距GM(1, 1)幂模型的特殊情形，幂指数的优化有利于提高建模精度。最后通过一个工程实例验证了非等间距GM(1, 1)幂模型的有效性与实用性。

关键词：灰色系统非等间距序列 GM(1 1)幂模型参数优化

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磁控溅射法生长的带隙可调谐(GaxIn1−x)2O3 Research Articles

张法碧1,孙巾寓1,李海鸥1,周娟1,王荣1,孙堂友1,傅涛1,肖功利1,李琦1,刘兴鹏1,张秀云1,郭道友2,王相虎3,秦祖军1

《信息与电子工程前沿（英文）》 2021年第22卷第10期页码 1370-1378 doi: 10.1631/FITEE.2000330

摘要：采用磁控溅射技术和热退火技术在(0001)蓝宝石衬底上制备了多组分氧化物(GaxIn1−x)2O3薄膜，实现可调带隙。详细研究了三元化合物(GaxIn1−x)2O3在整个组成范围内的光学性质和能带结构演化。X射线衍射谱表明，Ga含量在0.11至0.55之间的(GaxIn1−x)2O3薄膜既有立方结构，也有单斜结构，而Ga含量高于0.74的(GaxIn1−x)2O3薄膜只有单斜结构。实验结果为透明导电化合物半导体(GaxIn1−x)2O3薄膜在光电和光伏行业的应用，特别是在显示器、发光二极管和太阳能电池的应用奠定了基础。

关键词： (GaxIn1−x)2O3薄膜；带隙可调谐；磁控溅射

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Soluble triggering receptor expressed on myeloid cell-1 (sTREM-1): a potential biomarker for the diagnosis

Changlin Cao, Jingxian Gu, Jingyao Zhang

《医学前沿（英文）》 2017年第11卷第2期页码 169-177 doi: 10.1007/s11684-017-0505-z

摘要： Sensitive and useful biomarkers for the diagnosis and prognosis of infectious diseases have been widely developed. An example of these biomarkers is triggering receptor expressed on myeloid cell-1 (TREM-1), which is a cell surface receptor expressed on monocytes/macrophages and neutrophils. TREM-1 amplifies inflammation by activating the TREM-1/DAP12 pathway. This pathway is triggered by the interaction of TREM-1 with ligands or stimulation by bacterial lipopolysaccharide. Consequently, pro-inflammatory cytokines and chemokines are secreted. Soluble TREM-1 (sTREM-1) is a special form of TREM-1 that can be directly tested in human body fluids and well-known biomarker for infectious diseases. sTREM-1 level can be potentially used for the early diagnosis and prognosis prediction of some infectious diseases, including infectious pleural effusion, lung infections, sepsis, bacterial meningitis, viral infections (e.g., Crimean Congo hemorrhagic fever and dengue fever), fungal infections (e.g., infection), and burn-related infections. sTREM-1 is a more sensitive and specific biomarker than traditional indices, such as C-reactive protein and procalcitonin levels, for these infectious diseases. Therefore, sTREM-1 is a feasible biomarker for the targeted therapy and rapid and early diagnosis of infectious diseases.

关键词： soluble triggering receptor expressed on myeloid cells-1 infectious diseases diagnosis and prognosis biomarker

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Molecular characterization of two suppressor of cytokine signaling 1 genes (

Xue XU,Jiannan ZHANG,Juan LI,Yajun WANG

《农业科学与工程前沿（英文）》 2015年第2卷第1期页码 73-83 doi: 10.15302/J-FASE-2015044

摘要： Suppressor of cytokine signaling 1 (SOCS1) protein can inhibit the signal transduction triggered by some cytokines or hormones and thus are important in many physiological/pathological processes, including innate and adaptive immunity, inflammation, and development in mammals. However, there is sparse information about their structure, tissue expression, in birds, where their biological functions remain unknown. In this study, we cloned and characterized two genes (named and ) from chickens. is predicted to encode a 207-amino acid protein, which shares high amino acid sequence identity (64%–67%) with human and mouse SOCS1. Besides , a novel gene was also identified in chickens and other non-mammalian vertebrates including . Chicken is predicted to encode a 212-amino acid protein, which shares only 30%–32% amino acid sequence identity with human SOCS1 and cSOCS1a. RT-PCR assay revealed that both and are widely expressed in all chicken tissues. Using a luciferase reporter assay system, we further demonstrated that transient expression of and can significantly inhibit chicken growth hormone (GH)- or prolactin (PRL)-induced luciferase activities of Hep G2 cells expressing cGH receptor (or cPRL receptor), indicating that SOCS1a and SOCS1b proteins can negatively regulate GH/PRL signaling. Taken together, these data suggest that both cSOCS1a and cSOCS1b may function as negative regulators of cytokine/hormone actions, such as modulation of GH/PRL actions in chickens.

关键词： chicken SOCS1a SOCS1b growth hormone prolactin

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视觉知识引导的中国篆刻智能化生成 Research Article

张克俊1,2,张瑞1,殷叶航1,李一非3,伍文棋1,孙凌云1,2,吴飞1,邓晃煌1,潘云鹤1

《信息与电子工程前沿（英文）》 2022年第23卷第10期页码 1479-1493 doi: 10.1631/FITEE.2100094

摘要：

本文将传统篆刻艺术中的资源协同、设计创作、视觉呈现等过程以数字化方式再现，研制了篆刻艺术智能化创作的系统和平台（浙江大学智能篆刻系统：http://www. next.zju.edu.cn/seal/；篆刻搜索排版系统：http://www.next.zju.edu.cn/seal/search_app/），以视觉知识为引导突破计算机艺术学面临的难点问题。本文构建了包含字和印的求是篆刻数据库，并以此为视觉知识库，构建了篆字智能生成算法。此外，为创建印章布局，提出一种篆字变形算法调整印章字符，并结合视觉知识实现智能篆字布局，以实现智能结构。实验结果表明本文所提方法和系统可有效解决篆刻艺术生成中的难点问题，为篆刻艺术的守正与创新提供理论与应用借鉴。

关键词：篆刻；智能生成；深度学习；参数化模型；计算机艺术

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光致旋转技术综述 Review Article

朱琦1,李楠1,苏鹤鸣1,李文强1,胡慧珠1,2

《信息与电子工程前沿（英文）》 2022年第23卷第2期页码 171-185 doi: 10.1631/FITEE.2000338

摘要：光致旋转技术兴起于20世纪90年代。光镊为研究激光角动量提供了一个理想平台。近几十年来，光致旋转技术被广泛运用在光学微操控实验和生物与微流控领域。近年来，其在经典和量子物理领域的应用潜力引起人们广泛关注。本文回顾了光致旋转技术实验与应用进展。首先介绍了角动量的基本研究。其次，介绍了由轨道角动量引起的光致旋转技术的发展和应用，并给出自旋角动量的概念。最后，介绍了光致旋转技术在高真空光阱中的应用与前景。随着液体介质中精密光学操作技术的成熟，高真空光镊技术为高速微纳转子开辟了一条新道路。

关键词：光镊；光致旋转；角动量；微纳转子

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标题作者时间类型操作

Loss of monocarboxylate transporter 1 aggravates white matter injury after experimental subarachnoid

期刊论文

Cotransfecting norepinephrine transporter and vesicular monoamine transporter 2 genes for increased retention

null

期刊论文

Expression and function of DMT1 without IRE in C6 cells mediated by recombinant adenovirus

Xixun DU*, Huamin XU*, Hong JIANG, Jun WANG, Lei WANG, Junxia XIE

期刊论文

a novel K+ transporter gene in cotton